Proteomic analysis of Tunisian grapevine cultivar Razegui under salt stress.
Identifieur interne : 000046 ( Main/Exploration ); précédent : 000045; suivant : 000047Proteomic analysis of Tunisian grapevine cultivar Razegui under salt stress.
Auteurs : Neila Jellouli [Tunisie] ; Hatem Ben Jouira ; Houda Skouri ; Abdelwahed Ghorbel ; Ali Gourgouri ; Ahmed MlikiSource :
- Journal of plant physiology [ 0176-1617 ] ; 2008.
Descripteurs français
- KwdFr :
- Alignement de séquences (MeSH), Chlorure de sodium (pharmacologie), Données de séquences moléculaires (MeSH), Protéines végétales (analyse), Protéines végétales (génétique), Protéome (analyse), Protéome (génétique), Protéomique (méthodes), Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques), Séquence nucléotidique (MeSH), Vitis (effets des médicaments et des substances chimiques), Vitis (génétique), Vitis (métabolisme).
- MESH :
- analyse : Protéines végétales, Protéome.
- effets des médicaments et des substances chimiques : Régulation de l'expression des gènes végétaux, Vitis.
- génétique : Protéines végétales, Protéome, Vitis.
- métabolisme : Vitis.
- méthodes : Protéomique.
- pharmacologie : Chlorure de sodium.
- Alignement de séquences, Données de séquences moléculaires, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Gene Expression Regulation, Plant (drug effects), Molecular Sequence Data (MeSH), Plant Proteins (analysis), Plant Proteins (genetics), Proteome (analysis), Proteome (genetics), Proteomics (methods), Sequence Alignment (MeSH), Sodium Chloride (pharmacology), Vitis (drug effects), Vitis (genetics), Vitis (metabolism).
- MESH :
- chemical , analysis : Plant Proteins, Proteome.
- drug effects : Gene Expression Regulation, Plant, Vitis.
- chemical , genetics : Plant Proteins, Proteome, Vitis.
- metabolism : Vitis.
- methods : Proteomics.
- chemical , pharmacology : Sodium Chloride.
- Base Sequence, Molecular Sequence Data, Sequence Alignment.
Abstract
Salt stress is one of the major abiotic stresses in agriculture worldwide, especially in the Mediterranean area. We report here a proteomic approach to investigate the salt stress-responsive proteins in grapevine (Vitis vinifera). Two-dimensional electrophoresis (2-DE) was used to analyze the proteome of the salt-tolerant Tunisian grapevine cultivar Razegui, subjected to a supply of 100mm NaCl over 15d. Analysis of 2-DE gels derived from stressed plants revealed more than 800 reproducibly detected protein spots, with 48 proteins displaying a differential expression pattern, including 32 up-regulated, 9 down-regulated and 7 new protein spots induced after salt treatment. The presence of stress-responsive proteins in the different plant organs suggests that salt spreads systemically. Edman degradation analysis and database searching aided us in identifying a major protein GP. Database analysis revealed that this peptide has a 98% sequence similarity with a pathogenesis-related (PR) protein 10 (V. vinifera). A full-length cDNA encoding the GP protein was isolated from grapevine salt-stressed leaves and sequenced. The predicted protein contained 158 amino acids and showed 98% identity with PR10 protein of of V. vinifera (accession no. Cac16165).
DOI: 10.1016/j.jplph.2007.02.009
PubMed: 17942184
Affiliations:
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Le document en format XML
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PO Box 901, Hammam-Lif 2050, Tunisia. jel_neil@yahoo.fr</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>CBBC, Laboratoire de physiologie Moléculaire de la Vigne, Centre de Biotechnologie de Borj Cedria. 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We report here a proteomic approach to investigate the salt stress-responsive proteins in grapevine (Vitis vinifera). Two-dimensional electrophoresis (2-DE) was used to analyze the proteome of the salt-tolerant Tunisian grapevine cultivar Razegui, subjected to a supply of 100mm NaCl over 15d. Analysis of 2-DE gels derived from stressed plants revealed more than 800 reproducibly detected protein spots, with 48 proteins displaying a differential expression pattern, including 32 up-regulated, 9 down-regulated and 7 new protein spots induced after salt treatment. The presence of stress-responsive proteins in the different plant organs suggests that salt spreads systemically. Edman degradation analysis and database searching aided us in identifying a major protein GP. Database analysis revealed that this peptide has a 98% sequence similarity with a pathogenesis-related (PR) protein 10 (V. vinifera). A full-length cDNA encoding the GP protein was isolated from grapevine salt-stressed leaves and sequenced. The predicted protein contained 158 amino acids and showed 98% identity with PR10 protein of of V. vinifera (accession no. Cac16165).</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Ben Jouira, Hatem" sort="Ben Jouira, Hatem" uniqKey="Ben Jouira H" first="Hatem" last="Ben Jouira">Hatem Ben Jouira</name><name sortKey="Ghorbel, Abdelwahed" sort="Ghorbel, Abdelwahed" uniqKey="Ghorbel A" first="Abdelwahed" last="Ghorbel">Abdelwahed Ghorbel</name><name sortKey="Gourgouri, Ali" sort="Gourgouri, Ali" uniqKey="Gourgouri A" first="Ali" last="Gourgouri">Ali Gourgouri</name><name sortKey="Mliki, Ahmed" sort="Mliki, Ahmed" uniqKey="Mliki A" first="Ahmed" last="Mliki">Ahmed Mliki</name><name sortKey="Skouri, Houda" sort="Skouri, Houda" uniqKey="Skouri H" first="Houda" last="Skouri">Houda Skouri</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Jellouli, Neila" sort="Jellouli, Neila" uniqKey="Jellouli N" first="Neila" last="Jellouli">Neila Jellouli</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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